Bio-Rad Laboratories CFX Opus 96 Real-Time PCR System, 96-Well, 5-Color + FRET, with Laptop and CFX Maestro Software

Bio-Rad Laboratories CFX Opus 96 Real-Time PCR System, 96-Well, 5-Color + FRET, with Laptop and CFX Maestro Software
Product Specifications
BRAND  Bio-Rad
MODEL  CFX Opus 96 Real-Time PCR System
DESCRIPTION
The Bio-Rad CFX Opus 96 is a network-connected real-time PCR system featuring a fixed 96-well Peltier block and six-channel optical detection, providing 5-color plus FRET capability and multiplexing of up to five targets per well. Its high sensitivity detects single-copy target sequences across a ten-order dynamic range, with all-channel scan times of just 12 seconds and FAM/SYBR-only scans in 3 seconds. Designed for precise and efficient thermal performance, it delivers ±0.2 °C accuracy, ±0.3 °C well-to-well uniformity, ramp rates up to 5 °C/sec, and a programmable 1–24 °C gradient across the 30–100 °C range for optimized assay development.
ADDITIONAL INFORMATION
 
  • Sample Capacity: 96 wells
  • Sample Volume: 1–50 µL (10–50 µL recommended)
  • Communications: USB 2.0 or above, Ethernet, and Wi-Fi (adapter required; not included)
  • Touchscreen: Adjustable, 12–55° angle of rotation
  • Instrument Operating System: Windows 10 IoT
  • Power Requirements: 100–240 VAC, 50/60 Hz, 850 W
  • Fuses: F10 A, 250 V
  • Dimensions (W × D × H): 13 × 22 × 14 in (33 × 56 × 36 cm)
  • Weight: 48 lb (22 kg)
96-Well Reaction Block
  • Block Type: Fixed 96-well sample block with Peltier heating and cooling
  • Temperature Range: 4–100 °C
  • Lid Heating Range: 30–110 °C
  • Maximum Ramp Rate: 5 °C/sec
  • Average Ramp Rate: 3.3 °C/sec
  • Thermal Accuracy: ±0.2 °C
  • Thermal Uniformity: ±0.3 °C (maximum difference between wells ≤ 0.6 °C), measured 10 seconds after reaching target temperature
  • Gradient Temperature Range: 30–100 °C
  • Programmable Gradient Span: 1–24 °C
Optical Detection
  • Excitation Source: Six filtered LEDs
  • Detection System: Six filtered photodiodes
  • Wavelength Range: 450–730 nm
  • Detection Format: 5-color plus FRET
  • Multiplexing Capability: Up to five targets per well
  • All-Channel Scan Time: 12 seconds
  • Single-Channel Scan Time: 3 seconds (FAM/SYBR)
  • Dynamic Range: 10 orders of magnitude
  • Sensitivity: Detects a single copy of target sequence in human genomic DNA
Software and Connectivity
  • Software: Bio-Rad CFX Maestro Software
  • Software Configuration: Installed on the included Windows laptop and paired to the instrument's serial number
  • Control Verification: Software successfully detects and controls the instrument
  • Connectivity: USB, Ethernet, and BR.io cloud operation
  • Standalone Operation: Adjustable touchscreen allows operation independent of the connected PC
  • Commercial Advantage: CFX Maestro Software is separately licensed (Catalog No. 12013758); the included laptop is already configured to control this instrument.
Applications
  • Gene expression profiling
  • Allelic discrimination
  • Melt-curve analysis
  • High-resolution melt (HRM) workflows
  • Viral and pathogen quantitation
  • Biomarker validation
  • Quality control (QC) release testing
Included Accessories and Documentation
  • (1) Bio-Rad CFX Opus 96 Real-Time PCR System
  • (1) Windows laptop with Bio-Rad CFX Maestro Software installed and configured
  • (1) Laptop power supply
  • (1) USB interface cable
  • (1) USB hub
  • (1) USB mouse
  • (1) Instrument power cord
  • (1) Bio-Rad CFX Opus Quick Start Guide
  • (1) Bio-Rad CFX Real-Time PCR Systems Safety and Regulatory Compliance Guide
Functional Verification
  • Instrument successfully detected by CFX Maestro Software using serial number 795BR05251
  • Motorized lid successfully opened and closed through software control
  • Successfully initiated and ran a live protocol (PrimePCR.prcl)
  • Run Configuration: SYBR/FAM scan mode, 20 µL sample volume, 6-step / 39-cycle program
  • Verified Run Status: Running — Step 1 of 6, Preheating
  • Sample Block Temperature During Verification: 22.5 °C
  • Lid Heater During Verification: Active at 40 °C
Condition Notes
  • Overall Condition: Lightly used
  • Cosmetic Condition: Excellent condition for a 2022 instrument
  • Clean white and green housing with no visible marks or impact damage
  • Motorized lid moves smoothly along both guide rails
  • Clean 96-well block with no visible reagent residue, staining, corrosion, or bent wells
  • Block seating surface and gasket are intact

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