The spectrofluorometer is a complex device, which features photomultiplier tubes, a photodiode, and in some cases, a charge coupled device detector. The devices can include a number of different components. The systems can come with a variety of different options as well. Some of the other components that many are using with their spectrofluorometer include polarizers, cryostats, filter holders, LEDs, slide holders, manual slits, computer controlled slits, near infrared detectors, and more. Some of the options are user friendly and are perfect for research study.
How spectrofluorometers work
The device is capable of measuring the emission from the sample at a single wavelength, or it can perform a full scan. This will record the intensity against the wavelength. Another term for this is emission spectra. They utilize high intensity light sources and they then shoot as many photons as possible toward the sample. The more photons used the higher the number of excited molecules. This leads to better and more accurate results. The light will pass through a filter or a monochromator. This makes it easy to choose the wavelength you are interested in to be selected.
This instrument makes use of the fluorescent properties that are natural in some compounds as a means to provide data about their chemical environment and concentration. They are used in a number of different industries and labs today.